[Abstract] Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation.
Antibodies specific for Brd U can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA.
Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
The incorporation of Brd U is normally analyzed in flow cytometry by labelling with a conjugate anti-Brd U antibody and DNA dyes Propidium Iodide (PI) to perform cell cycle analysis.
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