The theme of this UV TALK LETTER is the instrument validation of UV-VIS spectrophotometers.
Instrument validation is essential for determining the condition of your instrument.
It can be seen from Table 1 that the generic term "performance" encompasses various conditions depending on the point of focus.Instrument validation of a spectrophotometer involves selecting the items required to manage and determine the status of the instrument from among these performance items, and verifying them. A deuterium lamp is known to exhibit sharp energy peaks (emission lines) at 656.1 nm and 486.0 nm wavelengths.The emission lines of a deuterium or low-pressure mercury lamp or the absorption peaks of an optical filter for wavelength calibration are generally used to verify the wavelength accuracy. Consequently, the instrument’s wavelength accuracy can be verified by measuring the energy spectrum of a deuterium lamp, investigating the wavelength of the peak near 656.1 nm, and then comparing its wavelength value to 656.1 nm.This UV TALK LETTER describes details about the instrument validation of UV-VIS spectrophotometers.A spectrophotometer shines light at various wavelengths onto the sample and investigates the degree of absorption, reflection, and transmission of the light to perform qualitative or quantitative analysis of the sample.
So, what sort of performance does a spectrophotometer offer?JIS K0115 "General rules for molecular absorptiometric analysis" prescribes the performance items that should be displayed by the instrument, as shown in Table 1.To state that such products meet the expected quality standards, it is important to verify that the plant, equipment, and operational procedures are free of problems.Verification procedures must be established to ensure that the details of the verification are consistent, regardless of who performs it.The series of processes from performing the verification according to a defined procedure to documenting the verification results is generally known as "validation".Validation can be performed on a diverse range of objects, from tangible items such as plant and equipment to intangible work procedures and processes.